[experimental Studies of Steroid Diabetes in Rats].

It is well known that adrenal corticosteroids have many biological effects on the carbohydrate metabolism. However, the mechanism of corticosteroids on carbohydrate metabolism has not been completely clarified and remains to be studied. In the present investigation, it was intended to study the effects of dietary carbohydrates on the experimental steroid diabetes induced by Ingles method in which 10 mg of cortisone was administered to rats daily. Furthermore, the effect of anabolic steroid (Oxymetholone-HMD) on steroid diabetes was studied. In this experiment, rats were divided into two groups, namely 1) rats fed with customary diet 2) rats forcedly fed with high carbohydrate diet. In the case of customary feeding, the rats were fed with 18.0 g of commerical diet (Oriental Co. LTD.) daily containing 9.47 g of carbohydrate, 0.99 g of fat and 4.36 g of protein. In the second group, they were forcedly fed by a gastric tube with the solution containing 17.08 g of carbohydrate, 2.76 g of fat and 5.94 g of protein. During the experimental period, fasting blood sugarlevel, urinary glucose excretion and body weight were determined. The glucose tolerance test was performed using 10 cc of 20% glucose solution just before, one week and two weeks after cortisone administration. The results of this experiment are summarized as follows : 1) In both customary and high carbohydrate diet groups, it was ascertained that the fasting blood sugar level increased late in theexperimental period (10 to 14 days after cortisone administration). The rise of blood sugar level was slight in the customary diet group, but in the high carbohydrate diet group a marked rise in the blood sugar level was noticed. The group fed with the high carbohydrate diet alone, showed a rise of fasting blood sugar level early in the experimental period (2 to 4 days) and then returned to normal late in the experimental period. 2) Urinary glucose was not shown in the customary diet group during the whole experimtal period in spite of the cortisone administration. In the group fed with high carbohydrate diet alone, urinary glucose was temporarily seen early in the experimental period. When cortisone was administered to the group fed with high carbohydrate diet, glucose excretion in the urine increased more remarkably late in the experimental period than in the rats fed with high cardohydrate diet alone.